国立大学法人大阪大学 Developmental Biology Research B6;129-Foxh1<tm1Hmd>/HmdRbrc The transcription factor Foxh1 (horkhead box H1) gene mediates signaling by transforming growth factor-β, Activin, and Nodal. Foxh1 protein interacts with phospho-Smad2 to regulate specific downstream targets of these signals. Mutation in Foxh1 results in impaired orientation of anterior-posterior axis and various defects of anterior streak and node. The flox allele of this strain was generated by Flp-mediated deletion of the neo cassette located between frt sites from the original targeting vector which was designed to insert a combined loxP site and loxP-frt-neo-frt cassette. Conditional Foxh1 deficient mice can be generated by crossing with tissue-specific Cre mice. This strain is useful for the study of Foxh1 gene in vivo. C（3〜6か月） Developed by Drs. Masamichi Yamamoto and Hiroshi Hamada at Osaka University in 2001. C57BL/6Cr and (129X1/SvJ x 129S1/Sv)F1-Kitl+ mixed background. B6;129-FoxH1flox/flox, FASTflox/flox, B6;129-FoxH1tm2Hh(B6;129-FoxH1flox/flox,FASTflox/flox)Knockout mice B6;129-FoxH1flox/flox, FASTflox/flox, B6;129-FoxH1tm2Hh(B6;129-FoxH1flox/flox,FASTflox/flox)Knockout mice FoxH1 の部位特異的な機能解析のための開発。2000年、山本正道先生・坂井靖夫先生・濱田博司先生、大阪大学大学院生命機能研究科が開発。 ES細胞（R1）より樹立。C57BL/6Jと交配。現在B6;129でB6 側に2世代目同士の交配。 繁殖効率：A（良好） 飼育は大ゲージ（約25cm × 40cm)にて16匹。交配はオス1匹に対してメス1〜2匹/週。 B6;129-FoxH1tm2Hh(B6;129-FoxH1flox/flox,FASTflox/flox)Knockout mice Osaka Univ. Hiroshi HAMADA R1 [(129X1/SvJ x 129S1/Sv)F1-Kitl<+>] FoxH1 の部位特異的な機能解析のための開発。2000年、山本正道先生・坂井靖夫先生・濱田博司先生、大阪大学大学院生命機能研究科が開発。ES細胞（R1）より樹立。C57BL/6Jと交配。現在B6;129でB6 側に2世代目同士の交配。 Sibling Mating (Homozygote x Homozygote)Homozygote mutant mice are normal and fertile. Necessary documents for ordering:<ol><li>Order form (<A HREF="https://mus.brc.riken.jp/ja/wp-content/uploads/form/form_4.docx">Japanese</A> / <A HREF="https://mus.brc.riken.jp/en/wp-content/uploads/form/form_b.docx">English</A>)</li><li>Category I MTA: MTA for distribution with RIKEN BRC (<A HREF="https://mus.brc.riken.jp/ja/wp-content/uploads/form/form_5.docx">Japanese</A> / <A HREF="https://mus.brc.riken.jp/en/wp-content/uploads/form/form_c.docx">English</A>)</li><li>Acceptance of responsibility for living modified organism (<A HREF="https://mus.brc.riken.jp/ja/wp-content/uploads/form/form_7.docx">Japanese</A> / <A HREF="https://mus.brc.riken.jp/en/wp-content/uploads/form/form_g.docx">English</A>)</li></ol> true <a href='https://brc.riken.jp/mus/pcr01230'>Genotyping protocol -PCR-</a> RBRC01230 phage P1 loxP sites, yeast FRT (flipase recombination target) site, mouse FoxH1 genomic DNA Sibling Mating (Homozygote x Homozygote) Homozygote mutant mice are normal and fertile. 濱田　博司 条件を付加する。<br>研究成果の公表にあたって譲渡者の指定する文献を引用する。Genes Dev., 15, 1242-1256 (2001). C (3-6 months) 繁殖効率：A（良好）飼育は大ゲージ（約25cm × 40cm)にて16匹。交配はオス1匹に対してメス1〜2匹/週。 Cre/loxP system B6;129-FoxH1flox/flox, FASTflox/flox B6;129、B6 側に2世代目同士の交配。FoxH1部位特異的な機能解析。 In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Genes Dev., 15, 1242-1256 (2001).